CURRICULUM VITAE
Xiaozhou Zhang, Ph.D.
WEBSITE: www.nicebio.com
E-mail: xiaozhouzhang @ hotmail.com
EDUCATION
Ph.D. Environmental Microbiology & Environmental Engineering, Nanjing Agricultural University, China, 2006
B.Eng. Food Science & Engineering, Laiyang Agricultural College, China, 2001
WORK EXPERIENCES
08/2008-Present	Postdoctoral Associate (Bioprocess Engineering), Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA
06/2006-08/2008	Postdoctoral Associate (Microbial Physiology and Genetics), Department of Biology, College of Arts and Sciences, Georgia State University, Atlanta, GA
01/2004-04/2006	Assistant Engineer, Center of Research & Design in Environmental and Energy Source Engineering, Nanjing Agricultural University, Nanjing, China
09/2001-05/2006	Graduate Research Assistant, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing, China
MEMBERDSHIPS TO SCIENTIFIC SOCIETIES
Sigma Xi, The Scientific Research Society, Full Membership American Society for Microbiology Chinese Society for Microbiology Chinese Society for Biogas
REVIEWER ACTIVITIES FOR INTERNATIONAL JOURNALS
Biodegradation Microbial Cell Factories Letters in Applied Microbiology Journal of Agricultural and Food Chemistry
LIST OF PUBLICATIONS
1. Xiao-Zhou Zhang, Xin Yan, Zhong-Li Cui, Qing Hong, Shun-Peng Li. mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis. Nucleic Acids Research, 2006, 34(9): e71. 2. Xiao-Zhou Zhang, Zhong-Li Cui, Qing Hong, Shun-Peng Li. High-level expression and secretion of methyl parathion hydrolase in Bacillus subtilis WB800. Applied and Environmental Microbiology, 2005, 71(7): 4101-4103. 3. Xiao-Zhou Zhang. Establishment and application of the new expression and genetic manipulation system in Bacillus subtilis. Ph.D. Dissertation, 2006, DOI:(CNKI:CDMD:1.2007.010271), Nanjing Agricultural University. 4. Xiao-Zhou Zhang, Jian-Hong Xu, Shun-Peng Li. Isolation, screening and preliminary identification of plant pathogenic fungi biocontrol strains of Bacillus spp. Soils, 2005, 37(1): 85-88. 5. Xiao-Zhou Zhang, Xin Yan, Zhong-Li Cui, Shun-Peng Li. Cloning of the strong promoters and signal peptide-encoding sequence from Brevibacillus brevis and construction of the shuttle expression-secretion vector. Acta Microbiologica Sinica, 2006, 46(4): 526-530. 6. Xiao-Zhou Zhang, Xin Yan, Zhong-Li Cui, Shun-Peng Li. Recombinant expression and secretion of mpd gene using the promoters and signal peptide-encoding sequences of ytkA and ywoF gene from Bacillus subtilis. Chinese Journal of Biotechnology, 2006, 22(2): 249-256. 7. Zhong-Li Cui, Xiao-Zhou Zhang, Zhong-Hui Zhang, Shun-Peng Li. Construction and application of a promoter trapping vector with methyl parathion hydrolase gene mpd as the reporter. Biotechnology Letters, 2004, 26(14): 1115-1118. 8. Zhi Liu, Xiao-Zhou Zhang, Jian He, Shao-Wen Lin, Jie-You Pan, Shun-Peng Li. Effects of nutrient substances and metal ions on degradation of p-nitrophenol by DLL-E4 (Pseudomonas putida). Acta Pedologica Sinica, 2004, 41(2): 170-175. 9. Zhi Liu, Xiao-Zhou Zhang, Shun-Peng Li. Use of methyl parathion (MP)-degrading strain DLL-E4 (Pseudomonas sp.) to remove MP residue on the surface of agriculture products. Chinese Journal of Applied Ecology, 2003, 14(9):1770-1774. 10. Xin Yan, Xiao-Zhou Zhang, Qing Hong, Jian He, Shun-Peng Li. A new method for the transfer of recombinant plasmids into some wildtype Bacillus strains. (Submitted) 11. Rui-Fu Zhang, Zhong-Li Cui, Xiao-Zhou Zhang, Jian-Dong Jiang, Ji-Dong Gu, Shunpeng Li. Cloning of the organophosphorus pesticide hydrolase gene clusters of seven degradative bacteria isolated from a methyl parathion contaminated site and evidence of their horizontal gene transfer. Biodegradation, 2006, 17(5): 465-472. 12. Zhong-Hui Zhang, Qing Hong, Jian-Hong Xu, Xiao-Zhou Zhang, Shun-Peng Li. Isolation of Fenitrothion-degrading strain Burkholderia sp. FDS-1 and Cloning of mpd Gene. Biodegradation, 2006, 17(3): 275-283. 13. Qing Hong, Zhong-Hui Zhang, Xiao-Zhou Zhang, Jian-Hong Xu, Shun-Peng Li. Cloning and sequencing of promoters of a moderate halophilic bacterium Halomonas sp. BY-1. Chinese Journal of Applied and Environmental Biology, 2005, 11(6): 729-732. 14. Zhi Liu, Ai-Zhi Ma, Xiao-Zhou Zhang, Xiao-Hua Zhang, Shun-Peng Li. Study on reaction system and characteristics of methyl parathion hydrolase. Journal of Nanjing Agricultural University, 2003, 26(10): 1770-1774. 15. Zhi Liu, Qing Hong, Xiao-Zhou Zhang, Jian-Hong Xu, Shun-peng Li. Character of p-nitrophenol degradation by methyl parathion degrading strain DLL-E4. China Environmental Science, 2003, 23(4): 435-439. 16. Guo-Shun Zhang, Qing Hong, Zhi Liu, Xiao-Zhou Zhang, Shun-Peng Li. Isolation and preliminary mechanism of acid tolerance of an acidphilic yeast strain R1. Acta Microbiologica Sinica, 2004, 44(5): 631-635. 17. Qing Hong, Jian-Hong Xu, Jun Wu, Xiao-Zhou Zhang, Shun-Peng Li. Cloning of a salt-tolerance related DNA fragment of a moderate halophilic bacterium Halomonas sp. BYS-1. Chinese Journal of Applied and Environmental Biology, 2005, 11(6): 729-732. 18. Zhi Liu, Qing Hong, Jian-Hong Xu, Jun Wu, Xiao-Zhou Zhang, Xiao-Hua Zhang, Ai-Zhi Ma, Jun Zhu, Shun-Peng Li. Cloning, analysis and fusion expression of methyl parathion hydrolase. Acta Genetica Sinica, 2003, 30(11): 1020-1026. 19. Guo-Shun Zhang, Qing Hong, Ai-Zhi Ma, Xiao-Zhou Zhang, Shun-Peng Li. Cloning, expression of the gene of dehydrochlorination of BHC from enrichment and its degradation experiment. Acta Microbiologica Sinica, 2005, 45(2): 44-47. 20. Jian-Dong Jiang, Rui-Fu Zhang, Jian He, Xiao-Zhou Zhang, Zhong-Li Cui, Shun-Peng Li. Bacterial chemotaxis to environmental pollutants and it’s significance in bioremediation. Acta Ecologica Sinica, 2005, 25(7): 1764-1771.
TECHNIQUES ACQUAINTED WITH
Molecular Biology:
DNA recombination, transformation, vector design and construction, isolation and purification of DNA, restriction mapping, library construction and gene cloning, Southern blotting, Western blotting, gene deletion and mutagenesis, site-directed mutagenesis, PCR, primers design.
Biochemistry:
Expression of foreign proteins in E. coli and B. subtilis, protein extraction and purification, enzyme assay, zymogram analysis, agarose electrophoresis, SDS-PAGE, chromatography techniques.
Bacteriological Techniques:
Sterilization methods, isolation, screening and identification of bacteria; inoculation and culture of bacteria, yeast and fungi, competent cells preparation.
Bioinformation and Computer Skills:
Skillful at computer operating system and hardware, imaging compile (PowerPoint, Photoshop), webpage design and construction, web-based database searches and sequence analysis, and major software for molecular biology and data analysis such as BioEdit, BandScan, DNAstar and Primer Premier.
MAIN RESEARCH FIELDS INVOLVED
Bioenergy, cellulase characterization & engineering, cellulose hydrolysis mechanisms, high-level protein expression and extracellular secretion in E. coli and B. subtilis; metabolic engineering for producing secondary metabolites. Investigation of the signal-less proteins secretion in bacteria. To elucidate the mechanisms of these proteins’ non-classical secretion and the relationship of their secretion with the pathogenesis. Development of the new target for the pathogentic bacteria’s control. The establishment and application of the new molecular cloning and high-efficiency expression and secretion system in Bacillus subtilis strains; the recombinant expression and secretion, separation and purification of the proteins in Bacillus subtilis; development of new homologous recombinant vectors which can be generally used in gram positive bacteria easily. Applied and environmental microbiology: the research of microbiological degradation of toxic organic pollutant. Isolation, screening and study of the characters of the bacteria strains from polluted soils and water for the biodegradation of organophosphorus pesticides; cloning and overexpression of the organophosphorus hydralase gene. The biodegradation and elimination of the pesticide residue from the contaminated environment and farm products. The construction and application of the new promoter-trapping vectors with the mpd (methyl parathion degrading) gene or gfp (green fluorescence protein) gene as the reporter; cloning the strong promoter and signal peptide fragments for the high-level expression and secretion of the foreign proteins. Plant pathogens biocontrol: Isolation, screening and identification of biocontrol bacteria strain from the collected soil samples for controlling the pathogenic fungi to the plant. Characterization and performance comparison of several Saccharomyces cerevisiae.
PRESENTATIONS, AWARDS AND HONORS
Oral Presentation and Honored with Excellent Paper on the Annual Academic Conference of Jiangsu Society for Microbiology, In Nov., 2005. Oral Presentation and Honored with Excellent Paper on the Annual Academic Conference of Jiangsu Society for Microbiology, In Nov., 2004. Oral Presentation and Honored with Excellent Paper on the Jiangsu Youth Microbiology Academic Conference, In Dec., 2003. Oral Presentation and Honored with Excellent Paper on the Jiangsu Province Doctoral Academic Conference, In Dec., 2004. Honored with the Outstanding Academia Model of Graduates of Nanjing Agricultral University, In Jun., 2005. Honored with the Outstanding Graduate of Nanjing Agricultral University, In Jun., 2006. Awarded with the Jin Shan-Bao Who’s Who Scholarship of Nanjing Agricultural University, In Dec., 2005. Awarded with the Fan Qing-Sheng Who’s Who Scholarship of Nanjing Agricultural University, In Dec., 2004.