| Research
Fields and Interests |
| Bioenergy,
cellulase characterization & engineering, cellulose hydrolysis
mechanisms, high-level protein expression and extracellular secretion
in E. coli and B. subtilis; metabolic engineering for producing secondary
metabolites. |
| The
establishment and application of the new molecular cloning and high-efficiency
expression and secretion system in Bacillus subtilis strains;
the recombinant expression and secretion, separation and purification
of the methyl parathion hydrolase in Bacillus subtilis; development
of new homologous recombinant vectors which can be generally used
in gram positive bacteria easily; development of new plasmid transfer
system between typical Bacillus subtilis and wildtype bacillus
isolates. |
| Applied
and environmental microbiology: the research of microbiological
degradation of toxic organic pollutant. Isolation, screening and
study of the characters of the bacteria strains from polluted soils
and water for the biodegradation of organophosphorus pesticides;
cloning and overexpression of the organophosphorus hydralase gene.
The biodegradation and elimination of the pesticide residue from
the contaminated environment and farm products. |
| The
construction and application of the promoter-trapping vectors with
the mpd (methyl parathion degrading) gene or gfp (green fluorescence
protein) gene as the reporter; cloning the strong promoter and signal
peptide fragments for the high-level expression and secretion of the
foreign proteins. |
| Isolation,
screening and identification of biocontrol bacteria strain from the
collected soil samples for controling the pathogenic fungi to the
plant. Four strains of Bacillus spp. have been isolated; experiments
show they all had strong antagonistic effects on a broad spectrum
of plant pathogenic fungi. |
| Characterization
and performance comparison of several Saccharomyces cerevisiae. |
