| CURRICULUM
VITAE |
| Family
name: Zhang |
| Given
name: Xiao-Zhou |
| Nationality:
Chinese |
| Mailing
address: Department of Microbiology, College of Life Sciences, Nanjing
Agricultural University, 6 Tongwei Road, Nanjing, Jiangsu Province
210095, P. R. China |
| Personal
Homepage: http://www.nicebio.com |
| E-mail
address: xiaozhouzhang @ hotmail.com |
| Education: |
Ph.D.
(2006) in Environmental Microbiology Engineering.
Department of Microbiology, College of Life Sciences, Nanjing Agricultural
University, Nanjing, China. (2001.09-2006.05) |
B.S.
(2001) in Food Science and Engineering.
Department of Food Science, Laiyang Agricultural College, Laiyang,
China. (1997.09-2001.07) |
| Memberships:
|
| Member,
Chinese Society for Microbiology |
| Research
Fields: |
| The
establishment and application of the new molecular cloning and high-efficiency
expression and secretion system in Bacillus subtilis strains;
the recombinant expression and secretion, separation and purification
of the methyl parathion hydrolase in Bacillus subtilis; development
of new homologous recombinant vectors which can be generally used
in gram positive bacteria easily; development of new plasmid transfer
system between typical Bacillus subtilis and wildtype bacillus
isolates. |
| Applied
and environmental microbiology: the research of microbiological
degradation of toxic organic pollutant. Isolation, screening and
study of the characters of the bacteria strains from polluted soils
and water for the biodegradation of organophosphorus pesticides;
cloning and overexpression of the organophosphorus hydralase gene.
The biodegradation and elimination of the pesticide residue from
the contaminated environment and farm products. |
| The
construction and application of the promoter-trapping vectors with
the mpd (methyl parathion degrading) gene or gfp (green fluorescence
protein) gene as the reporter; cloning the strong promoter and signal
peptide fragments for the high-level expression and secretion of the
foreign proteins. |
| Isolation,
screening and identification of biocontrol bacteria strain from the
collected soil samples for controling the pathogenic fungi to the
plant. Four strains of Bacillus spp. have been isolated; experiments
show they all had strong antagonistic effects on a broad spectrum
of plant pathogenic fungi. |
Techniques
Acquainted With: |
Molecular
Biology: |
| DNA
recombination, transformation, vector design and construction, isolation
and purification of DNA, restriction mapping, library construction
and gene cloning, Southern blotting, Western blotting, gene deletion
and mutagenesis, site-directed mutagenesis, PCR, primers design. |
Biochemistry: |
| Expression
of foreign proteins in E. coli and B. subtilis, protein extraction
and purification, enzyme assay, zymogram analysis, agarose electrophoresis,
SDS-PAGE, chromatography techniques. |
Bacteriological
Techniques: |
| Sterilization
methods, isolation, screening and identification of bacteria; inoculation
and culture of bacteria, yeast and fungi, competent cells preparation. |
Bioinformation
and Computer Skills: |
| Skillful
at computer operating system and hardware, imaging compile (PowerPoint,
Photoshop), webpage design and construction, web-based database searches
and sequence analysis, and major software for molecular biology and
data analysis such as BioEdit, BandScan, DNAstar and Primer Premier. |
English
Skills: |
| Passed
the National English Test CET-6. Excellent in reading and writing,
good at listening and speaking. |
| ¡¡ |
List
of Publications |
| Papers
in English: |
| Xiao-Zhou
Zhang, Zhong-Li Cui, Qing Hong, Shun-Peng Li. High-level
expression and secretion of methyl parathion hydrolase in Bacillus
subtilis WB800. Applied and Environmental Microbiology,
2005, 71(7): 4101-4103 |
| Xiao-Zhou
Zhang, Xin Yan, Zhong-Li Cui, Qing Hong, Shun-Peng
Li.A novel universal unmarked delivery system in Bacillus subtilis.Nucleic
Acids Research, 2006, (In Press)
|
| Zhong-Li
Cui, Xiao-Zhou Zhang, Zhong-Hui
Zhang, Shun-Peng Li. Construction and application of a promoter
trapping vector with methyl parathion hydrolase gene mpd
as the reporter. Biotechnology Letters,
2004, 26(14):1115-1118 |
| Zhong-Hui
Zhang, Qing Hong, Jian-Hong Xu, Xiao-Zhou Zhang,
Shun-Peng Li. Isolation of Fenitrothion-degrading strain Burkholderia
sp. FDS-1 and Cloning of mpd Gene. Biodegradation,
(In Press) |
| Rui-Fu
Zhang, Zhong-Li Cui, Xiao-Zhou Zhang,
Jian-Dong Jiang, Ji-Dong Gu, Shunpeng Li. Cloning of the organophosphorus
pesticide hydrolase gene clusters of seven degradative bacteria isolated
from a methyl parathion contaminated site and evidence of their horizontal
gene transfer. Biodegradation, (Accepted) |
| Papers
in Chinese: |
|
Xiao-Zhou Zhang, Jian-Hong Xu,
Shun-Peng Li. Isolation, screening and preliminary identification
of plant pathogenic fungi biocontrol strains of Bacillus
spp. Soils, 2005, 37(1):85-88 |
|
Xiao-Zhou Zhang, Xin
Yan, Zhong-Li Cui, Shun-Peng Li. Cloning of the strong promoters and
signal peptide-encoding sequence from Brevibacillus brevis and construction
of the shuttle expression-secretion vector. Acta Microbiologica Sinica,
(Accepted) |
| Xiao-Zhou
Zhang, Xin Yan, Zhong-Li Cui, Shun-Peng Li. Recombinant
expression and secretion of mpd gene using the promoters and signal
peptide-encoding sequences of ytkA and ywoF gene from Bacillus subtilis.
Chinese Journal of Biotechnology, (Accepted) |
| Zhi-Liu,
Xiao-Zhou Zhang, Jian He, Shao-Wen
Lin, Jie-You Pan, Shun-Peng Li. Effects of nutrient substances and
metal ions on degradation of p-nitrophenol by DLL-E4 (Pseudomonas
putida). Acta Pedologica Sinica, 2004, 41(2):170-175 |
| Zhi
Liu, Xiao-Zhou Zhang, Shun-Peng
Li. Use of methyl parathion (MP)-degrading strain DLL-E4 (Pseudomonas
sp.) to remove MP residue on the surface of agriculture products.
Chinese Journal of Applied Ecology, 2003, 14(9):1770-1774 |
|
Qing Hong, Zhong-Hui Zhang, Xiao-Zhou
Zhang, Jian-Hong Xu, Shun-Peng Li. Cloning and sequencing
of promoters of a moderate halophilic bacterium Halomonas sp. BY-1.
Chinese Journal of Applied and Environmental Biology, 2005, 11(6): |
| Zhi
Liu, Ai-Zhi Ma, Xiao-Zhou Zhang,
Xiao-Hua Zhang, Shun-Peng Li. Study on reaction system and characteristics
of methyl parathion hydrolase. Journal of Nanjing Agricultural University,
2003, 26(10):1770-1774 |
| Zhi
Liu, Qing Hong, Xiao-Zhou Zhang,
Jian-Hong Xu, Shun-peng Li. Character of p-nitrophenol degradation
by methyl parathion degrading strain DLL-E4. China Environmental
Science, 2003, 23(4):435-439 |
| Guo-Shun
Zhang, Qing Hong, Zhi Liu, Xiao-Zhou Zhang,
Shun-Peng Li. Isolation and preliminary mechanism of acid tolerance
of a acidphilic yeast strain R1. Acta Microbiologica Sinica, 2004,
44(5):631-635 |
| Zhi
Liu, Qing Hong, Jian-Hong Xu, Jun Wu, Xiao-Zhou
Zhang, Xiao-Hua Zhang, Ai-Zhi Ma, Jun Zhu, Shun-Peng
Li. Cloning, analysis and fusion expression of methyl parathion
hydrolase. Acta Genetica Sinica, 2003, 30(11):1020-1026 |
|
Guo-Shun Zhang, Qing Hong, Ai-Zhi Ma, Xiao-Zhou
Zhang, Shun-Peng Li. Cloning, expression of the gene
of dehydrochlorination of BHC from enrichment and its degradation
experiment. Acta Microbiologica Sinica, 2005, 45(2):44-47 |
|
Qing Hong, Jian-Hong Xu, Jun Wu, Xiao-Zhou
Zhang, Shun-Peng Li. Cloning of a salt-tolerance related
DNA fragment of a moderate halophilic bacterium Halomonas sp. BYS-1.
Chinese Journal of Applied and Environmental Biology, (Accepted) |
|
Jian-Dong Jiang, Rui-Fu Zhang, Jian He,
Xiao-Zhou Zhang, Zhong-Li Cui, Shun-Peng Li. Bacterial
chemotaxis to environmental pollutants and it¡¯s significance in bioremediation.
Acta Ecologica Sinica, 2005, 25(7):1764-1771 |
| Main
Awards and Honors: |
| Gained
the honor of the "Outstanding Academia Model of Nanjing Agricultral
University", In Jun., 2005 |
| Awarded
with the "Jin Shan-Bao Who¡¯s Who Scholarship" of Nanjing
Agricultural University, In Dec., 2005 |
| Won
the first award of the "Outstanding Postgraduate in the Midsemester
Examination ", In Jun., 2005 |
| Gained
the "Fan Qing-Sheng Who¡¯s Who Scholarship"
of Nanjing Agricultural University, In Dec., 2004 |
| Gained
the honor of the "Outstanding Postgraduate Both in Character
and Studies ", for twice, In Dec., 2002 and 2004 |
|
Won the second award of the "Outstanding Postgraduate in the
Midsemester Examination ", In Oct., 2003 |
| Gained
the honor of "Excellent Paper" in the 7th "Symposium
on Microbiology Research for the Youth in Jiangsu Province",
In Dec., 2004 |
| Gained
the honor of "Excellent Paper" in the 6th "Symposium
on Microbiology Research for the Youth in Jiangsu Province",
In Dec., 2003 |
| Gained
the honor of the "Outstanding Student" of Shandong province,
In Jun., 2000 |
| Won
the third prize of the 6th "Undergraduate's Festival for Science
and Technology, Culture and Art of Shandong Province" (Seals
Works Group), In May, 1999 |
| Gained
the "ZHENG-DA Scholarship" of Zheng-Da Co., Lit. Jinan,
Shandong province, for twice, In Oct., 1999 and 2000 |
|
